embryonic carcinoma nccit cells  (ATCC)

Journal: Cell Death Discovery

Article Title: Single-cell analysis unravels divergent gene signatures shaping seminoma stemness and metastasis

doi: 10.1038/s41420-025-02802-4

Figure Lengend Snippet: a The relationship between stemness-associated genes and pseudotime. b Microscopic images of NCCIT spheres after DPPA4 knockdown (scale bars: 100 µm (left); 50 µm (right)). Violin plot showing reduced sphere size and bar plot indicating decreased expression of stemness-related genes following DPPA4 knockdown. c Microscopic images of Tcam-2 spheres after DPPA4 knockdown. Violin plot showing smaller spheres and bar plot demonstrating reduced stemness-related gene expression after DPPA4 knockdown. d Microscopic images of NCCIT spheres after PSMA7 knockdown. Violin plot showing smaller spheres and bar plot indicating reduced stemness-related gene expression following PSMA7 knockdown. e Microscopic images of Tcam-2 spheres after PSMA7 knockdown. Violin plot showing reduced sphere size and bar plot showing decreased expression of stemness-related genes after PSMA7 knockdown. f Microscopic images of NCCIT spheres after PAGE5 overexpression. Violin plot showing smaller spheres and bar plot indicating reduced stemness-related gene expression following PAGE5 overexpression. g Microscopic images of Tcam-2 spheres after PAGE5 overexpression. Violin plot showing smaller spheres and bar plot demonstrating reduced stemness-related gene expression after PAGE5 overexpression. h Microscopic images of NCCIT spheres after SAT1 overexpression. Violin plot showing reduced sphere size and bar plot showing decreased expression of stemness-related genes following SAT1 overexpression. i Microscopic images of Tcam-2 spheres after SAT1 overexpression. Violin plot showing smaller spheres and bar plot demonstrating reduced stemness-related gene expression after SAT1 overexpression.

Article Snippet: The human embryonal carcinoma cell line NCCIT (ATCC, CRL-2073) and the seminoma cell line Tcam-2 (Otwo, Wuhan, China) were used in this study.

Techniques: Knockdown, Expressing, Gene Expression, Over Expression

Journal: Cell Death Discovery

Article Title: Single-cell analysis unravels divergent gene signatures shaping seminoma stemness and metastasis

doi: 10.1038/s41420-025-02802-4

Figure Lengend Snippet: a Confluence rate of Tcam-2 and NCCIT cells in wound healing assays (Tcam-2: Ctrl vs DPPA4 -KD: P < 0.01, Ctrl vs PSMA7 -KD: P < 0.05, Ctrl vs PAGE5 -OE: P < 0.01, Ctrl vs SAT1 -OE: P < 0.05; NCCIT: Ctrl vs DPPA4 -KD: P < 0.001, Ctrl vs PSMA7 -KD: P < 0.001, Ctrl vs PAGE5 -OE: P < 0.001, Ctrl vs SAT1 -OE: P < 0.05). b Box plot showing proliferation scores across different cell types. c GSEA plot showing enrichment of MYC target v1 gene signature in embryonal carcinoma cells compared to seminoma cells. d GSEA plot showing enrichment of MYC target v2 gene signature in embryonal carcinoma cells compared to seminoma cells. e GSEA plot showing enrichment of mTORC1 signaling gene signature in embryonal carcinoma cells compared to seminoma cells. f GSEA plot showing enrichment of MYC target v1 gene signature in metastatic seminoma cells compared to non-metastatic seminoma cells. g GSEA plot showing enrichment of MYC target v2 gene signature in metastatic seminoma cells compared to non-metastatic seminoma cells. h GSEA plot showing enrichment of mTORC1 signaling gene signature in metastatic seminoma cells compared to non-metastatic seminoma cells. i Growth curves of Tcam-2 and NCCIT cells (Tcam-2: Ctrl vs DPPA4 -KD: P < 0.01, Ctrl vs PSMA7 -KD: P < 0.01, Ctrl vs PAGE5 -OE: P < 0.0001, Ctrl vs SAT1 -OE: P < 0.0001; NCCIT: Ctrl vs DPPA4 -KD: P < 0.01, Ctrl vs PSMA7 -KD: P < 0.01, Ctrl vs PAGE5 -OE: P < 0.01, Ctrl vs SAT1 -OE: P < 0.01).

Article Snippet: The human embryonal carcinoma cell line NCCIT (ATCC, CRL-2073) and the seminoma cell line Tcam-2 (Otwo, Wuhan, China) were used in this study.

Techniques:

Journal: Molecular Biology Reports

Article Title: Expression of CD44 is associated with aggressiveness in seminomas

doi: 10.1007/s11033-024-09638-8

Figure Lengend Snippet: CD44 is highly expressed in TGCTs. ( A ) CD44 is overexpressed in TGCTs and distinct subtypes, seminomas and NSGCTs ( B ) compared to normal tissue. CD44 expression in 137 patients with TGCTs (68 seminomas and 68 NSGCTs) and 165 normal tissues were obtained by GEPIA2 (Gene Expression Profiling Interactive Analysis 2, http://gepia2.cancer-pku.cn/#index , TGCT dataset, match TCGA normal and GTEx data). ( C ) Relative mRNA levels of CD44 in JKT-1 seminoma, NCCIT and NTERA-2/D1(NT2/D1), embryonal carcinoma cells. The data are presented as the mean ± SD values ( n = 3). Asterisk (*) indicates statistically significant differences ( p ≤ 0.05) compared to JKT-1 cells. ( D ) Western blot analysis of protein levels of CD44 in JKT-1 seminoma, NCCIT and NTERA-2/D1(NT2/D1), embryonal carcinoma cells

Article Snippet: Mediastinal germ cell tumor embryonal carcinoma cell line NCCIT and NTERA-2/D1(NT2/D1) testicular embryonal carcinoma cell line were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Expressing, Gene Expression, Western Blot

Journal: Biomolecules & biomedicine

Article Title: Silencing of LINC00467 inhibits cell proliferation in testicular germ cell tumors cells.

doi: 10.17305/bb.2023.8969

Figure Lengend Snippet: Figure 1. Silencing of LINC00467 inhibited TGCT cell proliferation. Silencing efficiency of LINC00467 in TCam-2 (A) and NCCIT (B) cell lines; (C and D) The proliferation of cells in the NC group and LINC00467-silenced group (siRNA) was detected using an MTT assay; (E and F) The proliferation of cells in the NC group and LINC00467-silenced group was detected using CCK8 assay. *P value < 0.05; **P value < 0.01; ***P value < 0.001; ****P value < 0.0001. TGCT: Testicular germ cell tumor; siRNA: Small interfering RNA; NC: Negative control; CCK8: Cell Counting Kit 8.

Article Snippet: Tang Yuxin [19], while the non-seminoma human embryonic carcinoma cell line NCCIT was purchased from the American Type Culture Collection (ATCC) based in Virginia, USA.

Techniques: MTT Assay, CCK-8 Assay, Small Interfering RNA, Negative Control, Cell Counting

Journal: Biomolecules & biomedicine

Article Title: Silencing of LINC00467 inhibits cell proliferation in testicular germ cell tumors cells.

doi: 10.17305/bb.2023.8969

Figure Lengend Snippet: Figure 2. Silencing of LINC00467 inhibited TGCT cell cycle progression. (A) Determination of cell cycle distribution after LINC00467 silencing using flow cytometry. Cell cycle analysis of TCam-2 (B) and NCCIT (C). Error bars indicate SD (n = 3). *P value < 0.05; **P value < 0.01. TGCT: Testicular germ cell tumor; siRNA: Small interfering RNA; NC: Negative control.

Article Snippet: Tang Yuxin [19], while the non-seminoma human embryonic carcinoma cell line NCCIT was purchased from the American Type Culture Collection (ATCC) based in Virginia, USA.

Techniques: Cytometry, Cell Cycle Assay, Small Interfering RNA, Negative Control

Journal: Biomolecules & biomedicine

Article Title: Silencing of LINC00467 inhibits cell proliferation in testicular germ cell tumors cells.

doi: 10.17305/bb.2023.8969

Figure Lengend Snippet: Figure 3. LINC00467 silencing regulated cell cycle-related protein expression and inhibited the effect of DHT on cell proliferation. (A) Western blotting analysis of the change in the p21 and PCNA protein level after LINC00467 silencing. The relative expression of p21 and PCNA in each group was calculated using vinculin expression as an internal reference and normalized to one in the NC group of TCam-2 (B) and NCCIT (C); (D) DHT increased the expression of LINC00467; (E) The silencing of LINC00467 inhibited the effect of DHT on cell proliferation. **P value < 0.01; ***P value < 0.001; ****P value < 0.0001. DHT: Dihydrotestosterone; PCNA: Proliferating cell nuclear antigen; siRNA: Small interfering RNA; NC: Negative control; DMSO: Dimethylsulfoxide.

Article Snippet: Tang Yuxin [19], while the non-seminoma human embryonic carcinoma cell line NCCIT was purchased from the American Type Culture Collection (ATCC) based in Virginia, USA.

Techniques: Expressing, Western Blot, Small Interfering RNA, Negative Control